Anticorps Polyclonal de lapin anti-Pericentrin

Pericentrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 22271-1-AP

Synonymes

PCNT, Pericentrin-B, Pericentrin B

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Galerie de données de validation

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  IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 22271-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MDCK, cellules C2C12, cellules HeLa

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 4 publications below

Informations sur le produit

22271-1-AP cible Pericentrin dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: pericentrin
• Masse moléculaire calculée: 378 kDa
• Numéro d’acquisition GenBank: NM_006031
• Symbole du gène: Pericentrin
• Identification du gène (NCBI): 5116
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.

Publications

Species	Application	Title
human	IF	Elife; De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.; Authors - Won-Jing Wang
human	IF	Cell Death Differ; Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis; Authors - Haiyu Song
human	IF	Cell Rep; Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation; Authors - Zhenzhou Fang
mouse	IF	Sci Rep; Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition; Authors - Hannah C Saternos

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