Anticorps Polyclonal de lapin anti-Periostin

Periostin Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 27794-1-AP

Synonymes

OSF 2, OSF2, Osteoblast specific factor 2, PDLPOSTN, periostin, PN, POSTN, RP11 412K4.1

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Galerie de données de validation

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  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27794-1-AP cible Periostin dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Periostin Protéine recombinante Ag27145
• Nom complet: periostin, osteoblast specific factor
• Numéro d’acquisition GenBank: BC106710
• Symbole du gène: POSTN
• Identification du gène (NCBI): 10631
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.