Anticorps Monoclonal anti-Periostin

• Phare
• Validé par KD/KO

Periostin Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

1A11A3

N° de cat : 66491-1-PBS

Synonymes

1A11A3, OSF 2, OSF2, OSF-2, Osteoblast specific factor 2

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Galerie de données de validation

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  WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

  BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66491-1-Ig (same clone as 66491-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis using 66491-1-Ig (same clone as 66491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis using 66491-1-Ig (same clone as 66491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66491-1-Ig (same clone as 66491-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66491-1-Ig (same clone as 66491-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66491-1-PBS cible Periostin dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Periostin Protéine recombinante Ag14487
• Nom complet: periostin, osteoblast specific factor
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC106710
• Symbole du gène: Periostin
• Identification du gène (NCBI): 10631
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).

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