Anticorps Monoclonal anti-Peripherin

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H10B3

N° de cat : 66317-1-PBS

Synonymes

1H10B3, NEF4, PRPH

---

Galerie de données de validation

• 

  WB analysis using 66317-1-Ig (same clone as 66317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66317-1-Ig (same clone as 66317-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 66317-1-Ig (same clone as 66317-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 66317-1-Ig (same clone as 66317-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51128-2

  Cytometric bead array standard curve of MP51128-2, Peripherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66317-4-PBS. Detection antibody: 66317-1-PBS. Standard:Ag11116. Range: 0.781-100 ng/mL.

---

Informations sur le produit

66317-1-PBS cible Peripherin dans les applications de WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peripherin Protéine recombinante Ag11116
• Nom complet: peripherin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 50-54 kDa
• Numéro d’acquisition GenBank: BC032703
• Symbole du gène: Peripherin
• Identification du gène (NCBI): 5630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}