Anticorps Recombinant de lapin anti-Peripherin

Peripherin Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250661A3

N° de cat : 86057-1-PBS

Synonymes

NEF4, PRPH

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Galerie de données de validation

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  WB analysis using 86057-1-RR (same clone as 86057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86057-1-RR (Peripherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86057-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02242-2

  The mean Peripherin concentration was determined to be 2.78 ng/mL in COLO 320 cell extract based on a 1.7 mg/mL extract load and 124.43 ng/mL in IMR-32 extract based on a 2.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02242-2

  Sandwich ELISA standard curve of MP02242-2, Human Peripherin Recombinant Matched Antibody Pair - PBS only. 86057-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11365. 86057-1-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL

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  IHC staining of mouse colon using 86057-1-RR (same clone as 86057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86057-1-RR (Peripherin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86057-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 86057-1-RR (same clone as 86057-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Peripherin antibody (86057-1-RR, Clone: 250661A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86057-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02242-1

  The mean Peripherin concentration was determined to be 14.5 ng/mL in COLO 320 cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP02242-1

  Cytometric bead array standard curve of MP02242-1, Peripherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86057-2-PBS. Detection antibody: 86057-1-PBS. Standard: Ag11365. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86057-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86057-1-RR against Human Peripherin were performed. The affinity constant is 0.246 nM.

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Informations sur le produit

86057-1-PBS cible Peripherin dans les applications de WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peripherin Protéine recombinante Ag11365
• Nom complet: peripherin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC032703
• Symbole du gène: Peripherin
• Identification du gène (NCBI): 5630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

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