Anticorps Recombinant de lapin anti-Peroxiredoxin 2

Peroxiredoxin 2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251494D3

N° de cat : 86599-1-RR

Synonymes

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

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Galerie de données de validation

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  WB analysis using 86599-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86599-1-RR (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86599-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Peroxiredoxin 2 antibody (86599-1-RR, Clone: 251494D3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86599-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86599-1-RR against Human Peroxiredoxin 2 were performed. The affinity constant is 3.01 nM.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules C2C12, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris, tissu splénique de rat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86599-1-RR cible Peroxiredoxin 2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peroxiredoxin 2 Protéine recombinante Ag0835
• Nom complet: peroxiredoxin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003022
• Symbole du gène: peroxiredoxin 2
• Identification du gène (NCBI): 7001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxiredoxin 2 (PRDX2), also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

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