Anticorps Polyclonal de lapin anti-Phospho-ACC1 (Ser79)

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 29119-1-AP

Synonymes

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1, Phospho-ACC (Ser79)

---

Galerie de données de validation

• 

  WB analysis using 29119-1-AP

  Non-treated SH-SY5Y, phosphatase inhibitor treated and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

• 

  WB analysis using 29119-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y traitées à la λ phosphatase, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

29119-1-AP cible Phospho-ACC1 (Ser79) dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acetyl-Coenzyme A carboxylase alpha
• Masse moléculaire calculée: 2383 aa, 275 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC137287
• Symbole du gène: ACACA
• Identification du gène (NCBI): 31
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

Publications

Species	Application	Title
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
mouse	WB,IHC	Food Funct; Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.; Authors - Wei Tao
mouse	WB	Front Cardiovasc Med; MCC950, a Selective NLRP3 Inhibitor, Attenuates Adverse Cardiac Remodeling Following Heart Failure Through Improving the Cardiometabolic Dysfunction in Obese Mice.; Authors - Menglong Wang
human	WB	J Nat Prod; Hernandezine Regulates Proliferation and Autophagy-Induced Apoptosis in Melanoma Cells.; Authors - Xiaoting Wang
rat	WB	Antioxidants (Basel); 17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD); Authors - Ying Tian
rat	WB	Nutrients; Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats; Authors - Yujie Zhong