Anticorps Polyclonal de lapin anti-Phospho-ACC1 (Ser79)

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29119-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

  Non-treated SH-SY5Y, phosphatase inhibitor treated and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

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  WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29119-1-PBS cible Phospho-ACC1 (Ser79) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acetyl-Coenzyme A carboxylase alpha
• Masse moléculaire calculée: 2383 aa, 275 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC137287
• Symbole du gène: Acetyl-CoA Carboxylase 1
• Identification du gène (NCBI): 31
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

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