Anticorps Polyclonal de lapin anti-Phospho-AKT (Thr308)

Phospho-AKT (Thr308) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29163-1-PBS

Synonymes

AKT pan, AKT 1, AKT (pan), AKT, AKT1

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Galerie de données de validation

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  WB analysis using 29163-1-AP (same clone as 29163-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 29163-1-AP (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29163-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29163-1-PBS cible Phospho-AKT (Thr308) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)

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