Anticorps Recombinant de lapin anti-Phospho-AKT1 (Ser473)

Phospho-AKT1 (Ser473) Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

2M10

N° de cat : CL555-80462

Synonymes

AKT pan, AKT 1, AKT (pan), AKT, AKT1

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Galerie de données de validation

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  IF Staining of HeLa using CL555-80462

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite®555 Phospho-AKT1 (Ser473) antibody (CL555-80462, Clone: 2M10 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL555-80462

  1X10^6 NIH/3T3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human Phospho-AKT1 (Ser473) (CL555-80462, Clone:2M10), or 0.25 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A,
• Résultats positifs en FC (Intra): cellules NIH/3T3 traitées à la calyculine A

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-80462 cible Phospho-AKT1 (Ser473) dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: NM_005163
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80462-1-RR specifically recognizes AKT1 phosphorylated at Ser473.

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