Anticorps Recombinant de lapin anti-Phospho-AMPK Alpha (Thr172)

Phospho-AMPK Alpha (Thr172) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242923D4

N° de cat : 80209-6-RR

Synonymes

P-AMPK Alpha 2 (Thr172), Phos AMPK Alpha, Phos-AMPK Alpha 1 (Thr183), Phos-AMPK Alpha 2 (Thr172), Phospho-AMPK Alpha 1 (Thr183)

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Galerie de données de validation

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  WB analysis using 80209-6-RR

  Non-treated C2C12 cells and H2O2 treated C2C12 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: H2O2 treated C2C12 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80209-6-RR cible Phospho-AMPK Alpha (Thr172) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC048980
• Symbole du gène: AMPK Alpha 1
• Identification du gène (NCBI): 5562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMPK is a serine/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an "energy gauge". (PMID: 27034026, PMID: 21980456, PMID: 27600021)
This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.

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