Anticorps Recombinant de lapin anti-Phospho-AMPK Beta 1 (Ser182)

Phospho-AMPK Beta 1 (Ser182) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

240628B1

N° de cat : 83924-1-RR

Synonymes

AMPKβ1 (Ser182), AMPKβ1Ser182, p AMPK, p AMPK Beta 1, p AMPK Beta 1 (Ser182)

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Galerie de données de validation

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  WB analysis using 83924-1-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 83924-1-RR (Phospho-AMPK Beta 1 (Ser182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, λ phosphatase treated HEK-293 cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below

Informations sur le produit

83924-1-RR cible Phospho-AMPK Beta 1 (Ser182) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001007
• Symbole du gène: PRKAB1
• Identification du gène (NCBI): 5564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880). This antibody recognizes phosphorylated AMPK Beta 1.

Publications

Species	Application	Title
pig	WB	Front Nutr; Luteolin attenuates LPS-induced damage in IPEC-J2 cells by enhancing mitophagy via AMPK signaling pathway activation; Authors - Jianyun Yuan
human	WB	Anal Cell Pathol (Amst); Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis; Authors - Junli Zhang
human	WB	Cell Signal; Targeted therapy for KIF3C: A study on the mechanism of combined therapy with KIF3C signaling pathway, afatinib, and MT-DC (ac)phosphoramide in regulating gastric cancer cell proliferation; Authors - Maladho Tanta Diallo
mouse	WB	Nutrients; Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway; Authors - Qi Yan
mouse	WB	Biomaterials; Selective and iron-independent ferroptosis in cancer cells induced by manipulation of mitochondrial fatty acid oxidation; Authors - Yan Gao
mouse	WB	Diabetes Metab J; Shionone Inhibits Glomerular Fibirosis by Suppressing NLRP3 Related Inflammasome though SESN2-NRF2/ HO-1 Pathway; Authors - Tian Xiao

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