Anticorps Recombinant de lapin anti-Phospho-ASK1 (Ser83)

Phospho-ASK1 (Ser83) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241532B3

N° de cat : 80072-2-RR

Synonymes

P ASK1 (Ser83), P-ASK1 (Ser83), Phos ASK1 (Ser83), Phos-ASK1 (Ser83), ASK1

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Galerie de données de validation

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  WB analysis using 80072-2-RR

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80072-2-RR (Phospho-ASK1 (Ser83) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a control.

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  FC experiment of HEK-293 using 80072-2-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ASK1 (Ser83) Recombinant antibody (80072-2-RR, Clone:241532B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, λ phosphatase treated HEK-293 cells
• Résultats positifs en FC (Intra): λ phosphatase treated HEK-293 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80072-2-RR cible Phospho-ASK1 (Ser83) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase kinase 5
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC054503
• Symbole du gène: ASK1
• Identification du gène (NCBI): 4217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)

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