Anticorps Polyclonal de lapin anti-Phospho-ATF2 (Thr71)/ATF7 (Thr53)

Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28790-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28790-1-AP (same clone as 28790-1-PBS)

  Non-treated NIH/3T3 cells and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28790-1-AP (Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28790-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28790-1-PBS cible Phospho-ATF2 (Thr71)/ATF7 (Thr53) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: activating transcription factor 2
• Masse moléculaire calculée: 209 aa, 23 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC026175
• Symbole du gène: ATF2
• Identification du gène (NCBI): 1386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.

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