Anticorps Recombinant de lapin anti-Phospho-BAD (Ser112)

Phospho-BAD (Ser112) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251074F11

N° de cat : 80431-2-PBS

Synonymes

BAD (Ser112), p BAD (Ser112), p-BAD, p-BAD (Ser112), p-BAD(Ser112)

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Galerie de données de validation

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  WB analysis using 80431-2-RR (same clone as 80431-2-PBS)

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80431-2-RR (Phospho-BAD (Ser112) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80431-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80431-2-PBS cible Phospho-BAD (Ser112) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: BCL2-associated agonist of cell death
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC001901
• Symbole du gène: BAD
• Identification du gène (NCBI): 572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268)

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