Anticorps Recombinant de lapin anti-Phospho-CDK1 (Tyr15)

Phospho-CDK1 (Tyr15) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251130A5

N° de cat : 82751-5-PBS

Synonymes

cdc2 (Tyr15), CDK1 (Tyr15), p-cdc2, p-cdc2 (Tyr15), p-CDK1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 82751-5-RR (same clone as 82751-5-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 82751-5-RR (Phospho-CDK1 (Tyr15) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82751-5-PBS in a different storage buffer formulation.

---

Informations sur le produit

82751-5-PBS cible Phospho-CDK1 (Tyr15) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cell division cycle 2, G1 to S and G2 to M
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC014563
• Symbole du gène: CDK1
• Identification du gène (NCBI): 983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1. This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578)

{{ptg:RelatedPrimaryAntibodies}}