Anticorps Recombinant de lapin anti-Phospho-CHEK2 (Thr68)

Phospho-CHEK2 (Thr68) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1L2

N° de cat : 81740-1-RR

Synonymes

CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1

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Galerie de données de validation

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  WB analysis of PC-3 using 81740-1-RR

  Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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  WB analysis of HeLa using 81740-1-RR

  Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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  FC experiment of PC-3 using 81740-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.06 ug Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR, Clone:1L2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules PC-3 traitées au MMS, UV treated HeLa cells
• Résultats positifs en FC (Intra): cellules PC-3 traitées au MMS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

81740-1-RR cible Phospho-CHEK2 (Thr68) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: CHK2 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC004207
• Symbole du gène: CHEK2
• Identification du gène (NCBI): 11200
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Publications

Species	Application	Title
human	WB	EMBO Rep; SPIN1 facilitates chemoresistance and HR repair by promoting Tip60 binding to H3K9me3; Authors - Yukun Wang

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