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Anticorps Recombinant de lapin anti-Phospho-CHEK2 (Thr68)

Phospho-CHEK2 (Thr68) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1L2

N° de cat : CL594-81740

Synonymes

CHEK2 (Thr68), p CHEK2, p CHEK2 (Thr68), p CHEK2 Thr68, p-CHEK2

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®594-conjugated Phospho-CHEK2 (Thr68) Reco (CL594-81740)

    FC experiment of PC-3 using CL594-81740

    1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.25 ug Coralite®594 Phospho-Chek2 (Thr68) Recombinant Antibody (CL594-81740, Clone:1L2)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules PC-3 traitées au MMS,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-81740 cible Phospho-CHEK2 (Thr68) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet CHK2 checkpoint homolog (S. pombe)
Masse moléculaire calculée 61 kDa
Poids moléculaire observé65 kDa
Numéro d’acquisition GenBankBC004207
Symbole du gène CHEK2
Identification du gène (NCBI) 11200
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

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