Anticorps Recombinant de lapin anti-Phospho-Cardiac Troponin I (Ser23/24)

Phospho-Cardiac Troponin I (Ser23/24) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241982B1

N° de cat : 85002-1-RR

Synonymes

Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)

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Galerie de données de validation

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  WB analysis using 85002-1-RR

  Non-treated mouse heart tissue, phosphatase inhibitor treated mouse heart tissue and λ phosphatase treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 85001-1-RR (Phospho-Cardiac Troponin I (Ser23/24) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, λ phosphatase treated mouse heart tissue

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85002-1-RR cible Phospho-Cardiac Troponin I (Ser23/24) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: troponin I type 3 (cardiac)
• Masse moléculaire calculée: 210 aa, 24 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC096165
• Symbole du gène: Cardiac Troponin I
• Identification du gène (NCBI): 7137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

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