Anticorps Monoclonal anti-Phospho-Caspase 9 (Thr125)

Phospho-Caspase 9 (Thr125) Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5E11

N° de cat : 68136-1-PBS

Synonymes

CASP9, Caspase 9, Caspase 9/p35/p10, 1B5E11, APAF 3

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Galerie de données de validation

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  WB analysis using 68136-1-Ig (same clone as 68136-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 68136-1-Ig (Phospho-Caspase 9 (Thr125) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 68136-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68136-1-PBS cible Phospho-Caspase 9 (Thr125) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 9, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002452
• Symbole du gène: Caspase 9
• Identification du gène (NCBI): 842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46 kDa precursor protein which can be cleaved into a 35 kDa subunit and a 11 kDa subunit. Control of all caspases is tightly regulated by a series of phosphorylation events enacted by several different kinases. Caspase-9 is the most heavily phosphorylated of all caspases, with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507, PMID: 29066624)

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