Anticorps Recombinant de lapin anti-Phospho-Cofilin (Ser3)

Phospho-Cofilin (Ser3) Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250986H5

N° de cat : 86307-1-PBS

Synonymes

Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)

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Galerie de données de validation

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  WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

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  WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

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  IF Staining of Hela using 86307-1-RR (same clone as 86307-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated Hela cells using Phospho-Cofilin (Ser3) antibody (86307-1-RR, Clone: 250986H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86307-1-PBS cible Phospho-Cofilin (Ser3) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cofilin 1 (non-muscle)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC012318
• Symbole du gène: Cofilin
• Identification du gène (NCBI): 1072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

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