Anticorps Recombinant de lapin anti-Phospho-CrkL (Tyr207)

Phospho-CrkL (Tyr207) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240881A2

N° de cat : 84590-2-RR

Synonymes

CrkL (Tyr207), p CrkL, p CrkL (Tyr207), p-CrkL, p-CrkL (Tyr207)

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 84590-2-RR

  Non-treated NIH/3T3 cells and Blocked Phosphorylated peptide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-2-RR (Phospho-CrkL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, Blocked Phosphorylated peptide treated NIH/3T3 cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84590-2-RR cible Phospho-CrkL (Tyr207) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• Masse moléculaire calculée: 34 kDa
• Numéro d’acquisition GenBank: BC043500
• Symbole du gène: CRKL
• Identification du gène (NCBI): 1399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CrkL (v-crk avian sarcoma virus CT10 oncogene homolog-like) is a cytoplasmic adaptor protein that belongs to the Crk family of adaptors. Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)

{{ptg:RelatedPrimaryAntibodies}}