Anticorps Polyclonal de lapin anti-Phospho-EGFR (Tyr1069)
Phospho-EGFR (Tyr1069) Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 30277-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | EGF treated HeLa cells, |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 4 publications below |
IF | See 1 publications below |
Informations sur le produit
30277-1-AP cible Phospho-EGFR (Tyr1069) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
Masse moléculaire calculée | 1210 aa, 134 kDa |
Poids moléculaire observé | 170 kDa |
Numéro d’acquisition GenBank | BC094761 |
Symbole du gène | EGFR |
Identification du gène (NCBI) | 1956 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.
Protocole
Product Specific Protocols | |
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WB protocol for Phospho-EGFR (Tyr1069) antibody 30277-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cells CD133 Stimulates Cell Proliferation via the Upregulation of Amphiregulin in Melanoma | ||
IUBMB Life Tinagl1 restores tamoxifen sensitivity and blocks fibronectin-induced EMT by simultaneously blocking the EGFR and β1-integrin/FAK signaling pathways in tamoxifen-resistant breast cancer cells | ||
Life Sci Transcription factor PRRX1-activated ANXA6 facilitates EGFR-PKCα complex formation and enhances cisplatin sensitivity in bladder cancer | ||
Cytojournal Investigating the anti-cancer potential of sulfatase 1 and its underlying mechanism in non-small cell lung cancer |