Anticorps Recombinant de lapin anti-Phospho-EGFR (Tyr1197)

Phospho-EGFR (Tyr1197) Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241826G10

N° de cat : 84906-1-PBS

Synonymes

EGFR, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

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Galerie de données de validation

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  WB analysis using 84906-1-RR (same clone as 84906-1-PBS)

  Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84906-1-RR (Phospho-EGFR (Tyr1197) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84906-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84906-1-RR (same clone as 84906-1-PBS)

  1x10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-EGFR (Tyr1197) Recombinant Antibody (84906-1-RR, Clone:241826G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84906-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84906-1-PBS cible Phospho-EGFR (Tyr1197) dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Masse moléculaire calculée: 1210 aa, 134 kDa
• Poids moléculaire observé: 175 kDa
• Numéro d’acquisition GenBank: BC094761
• Symbole du gène: EGFR
• Identification du gène (NCBI): 1956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852)

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