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Anticorps Recombinant de lapin anti-Phospho-EGFR (Tyr1197)

Phospho-EGFR (Tyr1197) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

241826G10

N° de cat : CL594-84906

Synonymes

EGF Receptor (Tyr1173), EGFR (Tyr1197), p-EGF Receptor, p-EGF Receptor (Tyr1173), p-EGFR

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated Phospho-EGFR (Tyr1197) Rec (CL594-84906)

    FC experiment of A431 using CL594-84906

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Coralite®594 Phospho-EGFR (Tyr1197) Recombinant Antibody (CL594-84906, Clone:241826G10)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)EGF treated A431 cells,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-84906 cible Phospho-EGFR (Tyr1197) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Masse moléculaire calculée 1210 aa, 134 kDa
Poids moléculaire observé175 kDa
Numéro d’acquisition GenBankBC094761
Symbole du gène EGFR
Identification du gène (NCBI) 1956
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852)

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