Anticorps Recombinant de lapin anti-Phospho-EGFR (Tyr869)

Phospho-EGFR (Tyr869) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241785G5

N° de cat : 84839-1-RR

Synonymes

EGFR Tyr845, EGFR (Tyr845), p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845

---

Galerie de données de validation

• 

  WB analysis using 84839-1-RR

  Non-treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84839-1-RR (Phospho-EGFR (Tyr869) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

• 

  FC experiment of A431 using 84839-1-RR

  1x10^6 EGF treated A431 cells were intracellularly stained with 0.25 ug Phospho-EGFR (Tyr869) Recombinant Antibody (84839-1-RR, Clone:241785G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: EGF treated A431 cells,
• Résultats positifs en FC (Intra): EGF treated A431 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84839-1-RR cible Phospho-EGFR (Tyr869) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Masse moléculaire calculée: 1210 aa, 134 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC094761
• Symbole du gène: EGFR
• Identification du gène (NCBI): 1956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) This antibody recognizes EGFR phosphorylation at tyrosine 869, which is also widely described as the tyrosine 845 site.

{{ptg:RelatedPrimaryAntibodies}}