Anticorps Recombinant de lapin anti-Phospho-EIF2AK4 (Thr899)

Phospho-EIF2AK4 (Thr899) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

5N13

N° de cat : 82835-3-RR

Synonymes

EIF2AK4 (Thr899), EIF2AK4 Thr899, GCN2 (phospho T899), GCN2 (Thr899), p EIF2AK4

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Galerie de données de validation

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  WB analysis using 82835-3-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control.

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  WB analysis using 82835-3-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A, cellules NIH/3T3 traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82835-3-RR cible Phospho-EIF2AK4 (Thr899) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2 alpha kinase 4
• Masse moléculaire calculée: 187 kDa
• Poids moléculaire observé: 187 kDa
• Numéro d’acquisition GenBank: BC009350
• Symbole du gène: EIF2AK4
• Identification du gène (NCBI): 440275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4) belongs to a family of protein kinases that phosphorylate the α subunit of EIF2 (eukaryotic translation initiation factor 2) in response to various stress stimuli. Phosphorylation of EIF2A in response to EIF2AK4 activation subsequently increases translation of specific mRNAs that are important for stress remediation such as ATF4 (activating transcription factor 4). (PMID: 26986695)

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