Anticorps Monoclonal anti-Phospho-EIF2S1 (Ser51)

Phospho-EIF2S1 (Ser51) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

1A4A11

Avis (2)

N° de cat : 68023-1-Ig

Synonymes

EIF2S1 (Ser51), p EIF2S1, p EIF2S1 (Ser51), p EIF2S1 Ser51, p-EIF2S1

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Galerie de données de validation

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  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (HRP-66009) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis of HEK-293 using 68023-1-Ig

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 68023-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-EIF2S1 (Ser51) antibody (68023-1-Ig, Clone: 1A4A11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• 

  FC experiment of PC-3 using 68023-1-Ig

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-EIF2S1 (Ser51) (68023-1-Ig, Clone:1A4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293 traitées à la calyculine A, cellules HeLa, cellules HeLa traitées à la calyculine A, cellules HSC-T6, cellules HSC-T6 traitées à la calyculine A, cellules NIH/3T3, cellules NIH/3T3 traitées à la calyculine A, cellules PC-3 traitées à la calyculine A
• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A,
• Résultats positifs en FC (Intra): cellules PC-3 traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

68023-1-Ig cible Phospho-EIF2S1 (Ser51) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: NM_004094
• Symbole du gène: EIF2S1
• Identification du gène (NCBI): 1965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Publications

Species	Application	Title
human	WB,IF	Int Immunopharmacol; TRx0237 induces apoptosis and enhances anti-PD-1 immunotherapeutic efficacy in anaplastic thyroid Cancer; Authors - Qingyang Ning
human	WB	bioRxiv; Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells; Authors - Juan Jesus Vicente
human	WB	Cell Death Dis; AURKA emerges as a vulnerable target for KEAP1-deficient non-small cell lung cancer by activation of asparagine synthesis; Authors - Bing Deng
human	WB	J Transl Med; The microtubule targeting agent ST-401 triggers cell death in interphase and prevents the formation of polyploid giant cancer cells; Authors - Juan Jesus Vicente
human	WB	Environ Pollut; Copper exposure induces mitochondrial dysfunction and hepatotoxicity via the induction of oxidative stress and PERK/ATF4 -mediated endoplasmic reticulum stress; Authors - Meng Li
mouse,human	WB	Microbiol Res; Berberine ameliorates vascular dysfunction by downregulating TMAO-endoplasmic reticulum stress pathway via gut microbiota in hypertension; Authors - Zhichao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (09-15-2025)

Displays aspecific band and requires a bit of exposure using ECL and HRP-coupled secundary antibody but band is still visible

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: HCT116

Phospho-EIF2S1 (Ser51) Antibody Western Blot validation (1/2000 dilution) in HCT116 (Cat no:68023-1-Ig)

FH

Alla (Verified Customer) (07-24-2025)

Very good antibody.Have good image,but was not ale to uploade it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:12,000
• Cell Tissue Type: HEK 293

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