Anticorps Recombinant de lapin anti-Phospho-EIF4B (Ser406)

• Phare
• Validé par KD/KO

Phospho-EIF4B (Ser406) Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5G14

N° de cat : 80059-1-PBS

Synonymes

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

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Galerie de données de validation

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  WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

  WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 80059-1-RR (same clone as 80059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80059-1-PBS cible Phospho-EIF4B (Ser406) dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 4B
• Masse moléculaire calculée: 611 aa, 69 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC073154
• Symbole du gène: EIF4B
• Identification du gène (NCBI): 1975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).

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