Anticorps Recombinant de lapin anti-Phospho-EIF4E (Ser209)

Phospho-EIF4E (Ser209) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242049G10

N° de cat : 84981-1-RR

Synonymes

EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209

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Galerie de données de validation

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  WB analysis of HEK-293T using 84981-1-RR

  Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  FC experiment of HEK-293T using 84981-1-RR

  1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR, Clone:242049G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T traitées à la calyculine A,
• Résultats positifs en FC (Intra): cellules HEK-293T traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84981-1-RR cible Phospho-EIF4E (Ser209) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 4E
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC012611
• Symbole du gène: EIF4E
• Identification du gène (NCBI): 1977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)

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