Anticorps Recombinant de lapin anti-Phospho-ERK1/2 (Thr202/Tyr204)

Phospho-ERK1/2 (Thr202/Tyr204) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

8D12

N° de cat : CL647-80031

Synonymes

ERK (Thr202/Tyr204), ERK1/2 (Thr202/Tyr204), p ERK, p ERK (Thr202/Tyr204), p ERK1/2

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Galerie de données de validation

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  FC experiment of HepG2 using CL647-80031

  1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.5 ug Coralite® Plus 647 Phospho-Erk1/2 (Thr202/Tyr204) Recombinant Antibody (CL647-80031, Clone:8D12)(red), or 0.5 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en FC (Intra): cellules HepG2 traitées à la calyculine A,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-80031 cible Phospho-ERK1/2 (Thr202/Tyr204) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 3
• Masse moléculaire calculée: 38-43 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: NM_002746
• Symbole du gène: ERK1
• Identification du gène (NCBI): 5595
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.

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