Anticorps Recombinant de lapin anti-Phospho-ErbB3/HER3 (Tyr1289)

Phospho-ErbB3/HER3 (Tyr1289) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250208A12

N° de cat : 85053-6-PBS

Synonymes

ErbB3/HER3 (Tyr1289), HER3/ErbB3 (Tyr1289), p ErbB3/HER3 (Tyr1289), p HER3/ErbB3, p-ErbB3/HER3 (Tyr1289)

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Galerie de données de validation

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  WB analysis using 85053-6-RR (same clone as 85053-6-PBS)

  Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 85053-6-RR (Phospho-ErbB3/HER3 (Tyr1289) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85053-6-PBS in a different storage buffer formulation.

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Informations sur le produit

85053-6-PBS cible Phospho-ErbB3/HER3 (Tyr1289) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC002706
• Symbole du gène: ERBB3
• Identification du gène (NCBI): 2065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Unlike EGFR and HER2, their paralogue HER3 does not possess intrinsic kinase activity due to substitutions at critical positions in the kinase domain. To phosphorylate tyrosine residues in the HER3 cytoplasmic tail to recruit and activate intracellular signaling molecules, it must interact with another functional tyrosine kinase. HER3 exhibits this crosstalk preferentially with HER2 and EGFR, with which it can form heterodimers. Ligand-induced hetero-dimerization with EGFR or HER2 promotes HER3-tyrosine phosphorylation at 6 positions (1054, 1197, 1222, 1260, 1276, and 1289) in the C-terminal tail capable of recruiting of the SH2-domain-containing p85 activator of phosphatidyl-inositol-3 kinase (PI3K) and promoting activation of the downstream Akt signaling pathway. (PMID: 35249128)

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