Anticorps Recombinant de lapin anti-Phospho-Estrogen Receptor α (Ser118)

Phospho-Estrogen Receptor α (Ser118) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250278F8

N° de cat : 84564-6-PBS

Synonymes

ESR1, ER, ER alpha, Era, ER-alpha

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Galerie de données de validation

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  WB analysis using 84564-6-RR (same clone as 84564-6-PBS)

  T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

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Informations sur le produit

84564-6-PBS cible Phospho-Estrogen Receptor α (Ser118) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: estrogen receptor 1
• Masse moléculaire calculée: 595 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC128573
• Symbole du gène: ER
• Identification du gène (NCBI): 2099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)

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