Anticorps Polyclonal de lapin anti-Phospho-GSK3A (Tyr279)/GSK3B (Tyr216)

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (2)

N° de cat : 29125-1-AP

Synonymes

GSK3B (Tyr216), p GSK3B , p GSK3B (Tyr216), p GSK3B Tyr216, p-GSK3A (Tyr279)

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Galerie de données de validation

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  WB analysis using 29125-1-AP

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3B (Tyr216) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29125-1-AP

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules PC-3 traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• ELISA: See 1 publications below

Informations sur le produit

29125-1-AP cible Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glycogen synthase kinase 3 beta
• Masse moléculaire calculée: 433 aa, 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC000251
• Symbole du gène: GSK3B
• Identification du gène (NCBI): 2932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)

Publications

Species	Application	Title
mouse	WB	Exp Neurol; Inhibition of histamine receptor 3 alleviates sevoflurane-induced hypomyelination and neurobehavioral deficits; Authors - Ji Che
mouse	WB	Neurochem Res; Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes; Authors - Susu Lin
rat	WB	Evid Based Complement Alternat Med; Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3β Signaling Axis.; Authors - Hongcheng Zang
human	WB	Mar Drugs; Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism; Authors - Qianxia Lin
mouse	WB	Front Aging Neurosci; The γ-Adducin 1-357 fragment promotes tau pathology; Authors - Honglu Yu
mouse	WB	Aging (Albany NY); The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study; Authors - Yuanqing Cai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (06-25-2025)

Works nicely with my WB.

• Applications: Western Blot

FH

Patricia (Verified Customer) (01-31-2025)

Great Ab. Gave clean results.

• Applications: Western Blot

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