Anticorps Recombinant de lapin anti-Phospho-GYS (Ser641)

Phospho-GYS (Ser641) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250744A11

N° de cat : 80102-2-RR

Synonymes

GYS2, P GYS1 (Ser641), P-GYS1 (Ser641), Phos GYS1 (Ser641), Phos-GYS1 (Ser641)

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Galerie de données de validation

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  WB analysis using 80102-2-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80102-2-RR (Phospho-GYS (Ser641) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, λ phosphatase NIH/3T3 cells

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80102-2-RR cible Phospho-GYS (Ser641) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glycogen synthase 1 (muscle)
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC007688
• Symbole du gène: GYS1
• Identification du gène (NCBI): 2997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606)

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