Anticorps Recombinant de lapin anti-Phospho-HER2/ErbB2 (Tyr877)

Phospho-HER2/ErbB2 (Tyr877) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250539F5

N° de cat : 82762-11-RR

Synonymes

ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)

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Galerie de données de validation

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  WB analysis using 82762-11-RR

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82762-11-RR (Phospho-HER2/ErbB2 (Tyr877) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: EGF treated A431 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82762-11-RR cible Phospho-HER2/ErbB2 (Tyr877) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 1255 aa, 138 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC156755
• Symbole du gène: HER2/ErbB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)

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