Anticorps Recombinant de lapin anti-Phospho-HSP27 (Ser78)

Phospho-HSP27 (Ser78) Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241528D11

N° de cat : 80046-3-RR

Synonymes

Hsp27 (phospho S78), HSP27 (Ser78), HSP27-Ser78, Phospho HSP27 (Ser78), Phospho-HSP27

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Galerie de données de validation

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  WB analysis using 80046-3-RR

  Non treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80046-3-RR (Phospho-HSP27 (Ser78) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 80046-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-HSP27 (Ser78) antibody (80046-3-RR, Clone: 241528D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules PC-3 traitées à la calyculine A,
• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80046-3-RR cible Phospho-HSP27 (Ser78) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

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