Anticorps Recombinant de lapin anti-Phospho-Hamartin/TSC1 (Ser511)

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for Indirect ELISA, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

3N6

N° de cat : 80340-1-PBS

Synonymes

TSC1, TSC 1, TSC, LAM, KIAA0243

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Galerie de données de validation

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  WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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  WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

  Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80340-1-PBS cible Phospho-Hamartin/TSC1 (Ser511) dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 1
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000368
• Symbole du gène: Hamartin/TSC1
• Identification du gène (NCBI): 7248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).

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