Anticorps Recombinant de lapin anti-Phospho-Hamartin/TSC1 (Ser511)

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

3N6

N° de cat : 80340-1-RR

Synonymes

Hamartin, KIAA0243, LAM, Phospho tuberous sclerosis 1, Phospho-Hamartin/TSC1 (Ser511), TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

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Galerie de données de validation

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  WB analysis using 80340-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80340-1-RR

  Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Hela using 80340-1-RR

  1X10^6 Hela cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293 traitées à la calyculine A, cellules HeLa, cellules HeLa traitées à la calyculine A, cellules NIH/3T3 traitées à la calyculine A
• Résultats positifs en cytométrie: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80340-1-RR cible Phospho-Hamartin/TSC1 (Ser511) dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 1
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000368
• Symbole du gène: TSC1
• Identification du gène (NCBI): 7248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).