Anticorps Recombinant de lapin anti-Phospho-Histone H2A (Thr120)

Phospho-Histone H2A (Thr120) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250365B1

N° de cat : 83041-8-PBS

Synonymes

Histone H2A (Thr120), p Histone H2A, p Histone H2A (Thr120), p Histone H2A Thr120, p-Histone H2A

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Galerie de données de validation

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  WB analysis of HeLa using 83041-8-RR (same clone as 83041-8-PBS)

  Nocodazole and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 83041-8-RR (Phospho-Histone H2A (Thr120) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 83041-8-PBS in a different storage buffer formulation.

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Informations sur le produit

83041-8-PBS cible Phospho-Histone H2A (Thr120) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H2ae
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC093836
• Symbole du gène: HIST1H2AE
• Identification du gène (NCBI): 3012
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospho-Histone H2A (Thr120) refers to the phosphorylation of threonine 120 on histone H2A, a modification that is increasingly recognized for its roles in chromatin organization, gene regulation, and disease development, particularly in oncogenesis and bone remodeling. The phosphorylation is predominantly associated with gene silencing. High levels of H2AT120p correlate with increased cell proliferation, tumor growth, and poor prognosis, underscoring its role as an oncogenic signal.

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