Anticorps Recombinant de lapin anti-Phospho-Histone H2A (Thr120)

Phospho-Histone H2A (Thr120) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250365B1

N° de cat : 83041-8-RR

Synonymes

Histone H2A (Thr120), p Histone H2A, p Histone H2A (Thr120), p Histone H2A Thr120, p-Histone H2A

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Galerie de données de validation

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  WB analysis of HeLa using 83041-8-RR

  Nocodazole and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 83041-8-RR (Phospho-Histone H2A (Thr120) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

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Applications testées

• Résultats positifs en WB: Nocodazole and Calyculin A treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83041-8-RR cible Phospho-Histone H2A (Thr120) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H2ae
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC093836
• Symbole du gène: HIST1H2AE
• Identification du gène (NCBI): 3012
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phospho-Histone H2A (Thr120) refers to the phosphorylation of threonine 120 on histone H2A, a modification that is increasingly recognized for its roles in chromatin organization, gene regulation, and disease development, particularly in oncogenesis and bone remodeling. The phosphorylation is predominantly associated with gene silencing. High levels of H2AT120p correlate with increased cell proliferation, tumor growth, and poor prognosis, underscoring its role as an oncogenic signal.

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