Anticorps Polyclonal de lapin anti-Phospho-Histone H2A.X (Ser139)

Phospho-Histone H2A.X (Ser139) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 29380-1-AP

Synonymes

H2A.X (Ser139), H2A.X Ser139, Histone H2A.X (Ser139), Histone H2A.X Ser139, p Histone H2A.X

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Galerie de données de validation

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  WB analysis using 29380-1-AP

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29380-1-AP (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Histone H2A.X antibody and Histone H3 antibody as loading control.

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  IF Staining of U2OS using 29380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Phospho-Histone H2A.X (Ser139) antibody (29380-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Applications testées

• Résultats positifs en WB: UV treated HEK-293 cells,
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 4 publications below

Informations sur le produit

29380-1-AP cible Phospho-Histone H2A.X (Ser139) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: H2A histone family, member X
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC013416
• Symbole du gène: Histone H2A.X
• Identification du gène (NCBI): 3014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	J Nanobiotechnology; Synergistic cerium oxide nanozymes: targeting DNA damage and alleviating tumor hypoxia for improved NSCLC radiotherapy efficiency; Authors - Jie Liu
human	WB	Bioorg Chem; Dual inhibition of oxidative phosphorylation and glycolysis to enhance cancer therapy; Authors - Xi Sheng
mouse	IF	Ecotoxicol Environ Saf; 2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton; Authors - Chuanxin Zhang
human	WB	Eur J Med Chem; Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation; Authors - Amandeep Thakur
human	WB	Mol Cell; A TBK1-independent primordial function of STING in lysosomal biogenesis; Authors - Bo Lv

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