Anticorps Recombinant de lapin anti-Phospho-Histone H2A.X (Ser139)

Phospho-Histone H2A.X (Ser139) Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

5N19

N° de cat : 83307-2-RR

Synonymes

H2A.X (Ser139), H2A.X P-S139, H2A.X Ser139, H2A.XS139, H2A.XS139Ph

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Galerie de données de validation

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  WB analysis using 83307-2-RR

  Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control.

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  WB analysis of C6 using 83307-2-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of Jurkat using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Jurkat using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83307-2-RR

  Immunofluorescent analysis of (4% PFA) fixed UV treated HeLa cells using Phospho-Histone H2A.X (Ser139) antibody (83307-2-RR, Clone: 5N19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83307-2-RR

  1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-Histone H2A.X (Ser139) Recombinant antibody (83307-2-RR, Clone:5N19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la staurosporine, cellules C6
• Résultats positifs en IHC: cellules Jurkat, tissu splénique de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: UV treated HeLa cells,
• Résultats positifs en FC (Intra): cellules Jurkat traitées à la staurosporine,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

83307-2-RR cible Phospho-Histone H2A.X (Ser139) dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: H2A histone family, member X
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC013416
• Symbole du gène: Histone H2A.X
• Identification du gène (NCBI): 3014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Publications

Species	Application	Title
human	WB	Transl Oncol; PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7; Authors - Hanlin Hu
human	IF	J Microbiol Biotechnol; BlingLife®-Marigold Extract Alleviates Blue Light-Induced Retinal Mitochondria Oxidative Stress and Senescence by Activating NRF2/HO-1 Signaling; Authors - Zixiu Zhou

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