Anticorps Monoclonal anti-Phospho-Histone H3 (Ser10)

Phospho-Histone H3 (Ser10) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4C7G2

N° de cat : 66863-1-PBS

Synonymes

H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3

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Galerie de données de validation

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  WB analysis using 66863-1-Ig (same clone as 66863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  WB analysis using 66863-1-Ig (same clone as 66863-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 66863-1-Ig (same clone as 66863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 66863-1-Ig (same clone as 66863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66863-1-Ig (same clone as 66863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)

  1x10^6 nocodazole treated HeLa cells were intracellularly stained with 0.25 ug Phospho-Histone H3 (Ser10) Monoclonal antibody (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), and 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50178-1

  Cytometric bead array in cell lysate using MP50178-1, Phospho-Histone H3 (Ser10) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68345-1-PBS. Detection antibody: 66863-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Informations sur le produit

66863-1-PBS cible Phospho-Histone H3 (Ser10) dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: NM_003529
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

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