Anticorps Recombinant de lapin anti-Phospho-Histone H3 (Ser10)

Phospho-Histone H3 (Ser10) Recombinant Antibody for WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

242921B1

N° de cat : 82828-10-RR

Synonymes

H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3

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Galerie de données de validation

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  WB analysis of HeLa using 82828-10-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

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  WB analysis of NIH/3T3 using 82828-10-RR

  Nocodazole treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  WB analysis of HSC-T6 using 82828-10-RR

  Calyculin A treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  IHC staining of mouse brain using 82828-10-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 82828-10-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

• 

  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

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  ChIP experiment of HeLa using 82828-10-RR

  Chromatin was prepared from HeLa cells either non-treated or treated with Nocodazole (100 ng/ml, 16 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser10) (82828-10-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: nocodazole treated HeLa cells, cellules HSC-T6 traitées à la calyculine A
• Résultats positifs en IHC: tissu cérébral de souris, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en ChIP-qPCR: Nocodazole (100 ng/ml, 16 h) HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82828-10-RR cible Phospho-Histone H3 (Ser10) dans les applications de WB, IHC, IF/ICC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_003529
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

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