Anticorps Recombinant de lapin anti-Phospho-Histone H3 (Ser28)

Phospho-Histone H3 (Ser28) Recombinant Antibody for WB, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

250369A1

N° de cat : 82828-15-RR

Synonymes

Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28, p-Histone H3

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Galerie de données de validation

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  WB analysis of HeLa using 82828-15-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-15-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

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  ChIP experiment of HeLa using 82828-15-RR

  Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser28) (82828-15-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: nocodazole treated HeLa cells,
• Résultats positifs en ChIP-qPCR: Nocodazole-HeLa (100 ng/ml, 16 h) HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82828-15-RR cible Phospho-Histone H3 (Ser28) dans les applications de WB, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histone H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and β-globin genes (PMID: 17913747).

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