Anticorps Recombinant de lapin anti-Phospho-IRAK4 (Thr345/Ser346)

Phospho-IRAK4 (Thr345/Ser346) Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242271A8

N° de cat : 85753-1-PBS

Synonymes

IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346)

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Galerie de données de validation

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  WB analysis of Karpas-299 using 85753-1-RR (same clone as 85753-1-PBS)

  Non-treated Karpas-299 cells and IL-1 beta treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85753-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lymph node using 85753-1-RR (same clone as 85753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lymph node tissue slide using 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85753-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lymph node using 85753-1-RR (same clone as 85753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lymph node tissue slide using 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85753-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85753-1-PBS cible Phospho-IRAK4 (Thr345/Ser346) dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interleukin-1 receptor-associated kinase 4
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC013316
• Symbole du gène: IRAK4
• Identification du gène (NCBI): 51135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)

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