Anticorps Recombinant de lapin anti-Phospho-IRF3 (Ser396)

Phospho-IRF3 (Ser396) Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241522A6

N° de cat : 80519-2-PBS

Synonymes

IRF3, IRF-3, IRF 3, interferon regulatory factor 3, 241522A6

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Galerie de données de validation

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  WB analysis of THP-1 using 80519-2-RR (same clone as 80519-2-PBS)

  Phosphatase inhibitor treated or λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80519-2-RR (Phospho-IRF3 (Ser396) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80519-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 80519-2-RR (same clone as 80519-2-PBS)

  1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.25 ug Phospho-IRF3 (Ser396) Recombinant antibody (80519-2-RR, Clone:241522A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80519-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80519-2-PBS cible Phospho-IRF3 (Ser396) dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interferon regulatory factor 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC009395
• Symbole du gène: IRF3
• Identification du gène (NCBI): 3661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

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