Anticorps Recombinant de lapin anti-Phospho-IkB Alpha (Ser32/36)

Phospho-IkB Alpha (Ser32/36) Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1G21

N° de cat : 82349-1-PBS

Synonymes

p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α, p IkB α (Ser32/36)

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Galerie de données de validation

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  WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 82349-1-RR (same clone as 82349-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-IkB Alpha (Ser32/36) Recombinant antibody (82349-1-RR, Clone:1G21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

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Applications publiées

• WB: See 1 publications below

Informations sur le produit

82349-1-PBS cible Phospho-IkB Alpha (Ser32/36) dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004983
• Symbole du gène: IkB Alpha
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).

Publications

Species	Application	Title
mouse	WB	Neoplasia; ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation; Authors - Li-Quan Huang

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