Anticorps Recombinant de lapin anti-Phospho-IkB Beta (Thr19/Ser23)

Phospho-IkB Beta (Thr19/Ser23) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241061B2

N° de cat : 84403-1-RR

Synonymes

IkB beta, IκB beta, IκB β, NFKBIB, IkB B

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Galerie de données de validation

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  WB analysis of HT-29 using 84403-1-RR

  Non-treated HT-29 cells and TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84403-1-RR (Phospho-IkB Beta (Thr19/Ser23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  FC experiment of HT-29 using 84403-1-RR

  1x10^6 TNF alpha and Calyculin A treated or untreated(black) HT-29 cells were intracellularly stained with 0.25 ug Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody (84403-1-RR, Clone:241061B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: TNF alpha and Calyculin A treated HT-29 cells,
• Résultats positifs en FC (Intra): TNF alpha and Calyculin A treated HT-29 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

84403-1-RR cible Phospho-IkB Beta (Thr19/Ser23) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• Masse moléculaire calculée: 356 aa, 38 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015528
• Symbole du gène: IkB Beta
• Identification du gène (NCBI): 4793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.

Publications

Species	Application	Title
rat	WB	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu

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